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P Stat6 Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p-stat6 #9361 antibody  (Cell Signaling Technology Inc)
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Mechanistic exploration of diabetic wound healing facilitated by LZM-HZ hydrogels. (A) Volcano plot of differentially expressed genes (DEGs) between the LZM-HZ-10 hydrogels and blank on Day 7 highlights significant gene regulation differences. (B) Gene set enrichment analysis (GSEA) shows that DEGs are primarily involved in immune regulation pathways. (C) Heatmap of gene expression reveals the upregulation and downregulation of genes associated with diabetic wound healing in the LZM-HZ-10 hydrogels compared to the blank. (D) Gene Ontology (GO) enrichment analysis barplot illustrates the assessment enriched biological processes, with bar length indicating the number of genes and color representing the significance level (lighter colors denote higher significance). (E) KEGG pathway enrichment bubble chart showing the distribution of gene enrichment differences within pathways. While color reflects the p-value, with lighter shades indicating higher significance. (F) Representative Western blot bands of phosphorylated STAT3 (p-STAT3), phosphorylated <t>STAT6</t> (p-STAT6), and Arg1 in wound tissues from diabetic mice treated with LZM-HZ-10 hydrogel, LZM-SC hydrogel and Blank. (G–I) Quantitative analysis of relative protein expression levels normalized to GAPDH. Increased levels of p-STAT3, p-STAT6, and Arg1 in the LZM-HZ-10 hydrogel confirm activation of the JAK-STAT signaling pathway at the protein level. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.)
P Stat6 #9361 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p-stat6 (y641) ap1390 antibody  (ABclonal Biotechnology)
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Mechanistic exploration of diabetic wound healing facilitated by LZM-HZ hydrogels. (A) Volcano plot of differentially expressed genes (DEGs) between the LZM-HZ-10 hydrogels and blank on Day 7 highlights significant gene regulation differences. (B) Gene set enrichment analysis (GSEA) shows that DEGs are primarily involved in immune regulation pathways. (C) Heatmap of gene expression reveals the upregulation and downregulation of genes associated with diabetic wound healing in the LZM-HZ-10 hydrogels compared to the blank. (D) Gene Ontology (GO) enrichment analysis barplot illustrates the assessment enriched biological processes, with bar length indicating the number of genes and color representing the significance level (lighter colors denote higher significance). (E) KEGG pathway enrichment bubble chart showing the distribution of gene enrichment differences within pathways. While color reflects the p-value, with lighter shades indicating higher significance. (F) Representative Western blot bands of phosphorylated STAT3 (p-STAT3), phosphorylated <t>STAT6</t> (p-STAT6), and Arg1 in wound tissues from diabetic mice treated with LZM-HZ-10 hydrogel, LZM-SC hydrogel and Blank. (G–I) Quantitative analysis of relative protein expression levels normalized to GAPDH. Increased levels of p-STAT3, p-STAT6, and Arg1 in the LZM-HZ-10 hydrogel confirm activation of the JAK-STAT signaling pathway at the protein level. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.)
P Stat6 (Y641) Ap1390 Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mechanistic exploration of diabetic wound healing facilitated by LZM-HZ hydrogels. (A) Volcano plot of differentially expressed genes (DEGs) between the LZM-HZ-10 hydrogels and blank on Day 7 highlights significant gene regulation differences. (B) Gene set enrichment analysis (GSEA) shows that DEGs are primarily involved in immune regulation pathways. (C) Heatmap of gene expression reveals the upregulation and downregulation of genes associated with diabetic wound healing in the LZM-HZ-10 hydrogels compared to the blank. (D) Gene Ontology (GO) enrichment analysis barplot illustrates the assessment enriched biological processes, with bar length indicating the number of genes and color representing the significance level (lighter colors denote higher significance). (E) KEGG pathway enrichment bubble chart showing the distribution of gene enrichment differences within pathways. While color reflects the p-value, with lighter shades indicating higher significance. (F) Representative Western blot bands of phosphorylated STAT3 (p-STAT3), phosphorylated <t>STAT6</t> (p-STAT6), and Arg1 in wound tissues from diabetic mice treated with LZM-HZ-10 hydrogel, LZM-SC hydrogel and Blank. (G–I) Quantitative analysis of relative protein expression levels normalized to GAPDH. Increased levels of p-STAT3, p-STAT6, and Arg1 in the LZM-HZ-10 hydrogel confirm activation of the JAK-STAT signaling pathway at the protein level. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.)
P Stat6, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary antibodies against p-stat6  (ABclonal Biotechnology)
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Mechanistic exploration of diabetic wound healing facilitated by LZM-HZ hydrogels. (A) Volcano plot of differentially expressed genes (DEGs) between the LZM-HZ-10 hydrogels and blank on Day 7 highlights significant gene regulation differences. (B) Gene set enrichment analysis (GSEA) shows that DEGs are primarily involved in immune regulation pathways. (C) Heatmap of gene expression reveals the upregulation and downregulation of genes associated with diabetic wound healing in the LZM-HZ-10 hydrogels compared to the blank. (D) Gene Ontology (GO) enrichment analysis barplot illustrates the assessment enriched biological processes, with bar length indicating the number of genes and color representing the significance level (lighter colors denote higher significance). (E) KEGG pathway enrichment bubble chart showing the distribution of gene enrichment differences within pathways. While color reflects the p-value, with lighter shades indicating higher significance. (F) Representative Western blot bands of phosphorylated STAT3 (p-STAT3), phosphorylated <t>STAT6</t> (p-STAT6), and Arg1 in wound tissues from diabetic mice treated with LZM-HZ-10 hydrogel, LZM-SC hydrogel and Blank. (G–I) Quantitative analysis of relative protein expression levels normalized to GAPDH. Increased levels of p-STAT3, p-STAT6, and Arg1 in the LZM-HZ-10 hydrogel confirm activation of the JAK-STAT signaling pathway at the protein level. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.)
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antibody against phospho-stat6 (p-stat6, ap0456)  (ABclonal Biotechnology)
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Mechanistic exploration of diabetic wound healing facilitated by LZM-HZ hydrogels. (A) Volcano plot of differentially expressed genes (DEGs) between the LZM-HZ-10 hydrogels and blank on Day 7 highlights significant gene regulation differences. (B) Gene set enrichment analysis (GSEA) shows that DEGs are primarily involved in immune regulation pathways. (C) Heatmap of gene expression reveals the upregulation and downregulation of genes associated with diabetic wound healing in the LZM-HZ-10 hydrogels compared to the blank. (D) Gene Ontology (GO) enrichment analysis barplot illustrates the assessment enriched biological processes, with bar length indicating the number of genes and color representing the significance level (lighter colors denote higher significance). (E) KEGG pathway enrichment bubble chart showing the distribution of gene enrichment differences within pathways. While color reflects the p-value, with lighter shades indicating higher significance. (F) Representative Western blot bands of phosphorylated STAT3 (p-STAT3), phosphorylated <t>STAT6</t> (p-STAT6), and Arg1 in wound tissues from diabetic mice treated with LZM-HZ-10 hydrogel, LZM-SC hydrogel and Blank. (G–I) Quantitative analysis of relative protein expression levels normalized to GAPDH. Increased levels of p-STAT3, p-STAT6, and Arg1 in the LZM-HZ-10 hydrogel confirm activation of the JAK-STAT signaling pathway at the protein level. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.)
Antibody Against Phospho Stat6 (P Stat6, Ap0456), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit monoclonal anti p stat6 tyr641  (Cell Signaling Technology Inc)
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Mechanistic exploration of diabetic wound healing facilitated by LZM-HZ hydrogels. (A) Volcano plot of differentially expressed genes (DEGs) between the LZM-HZ-10 hydrogels and blank on Day 7 highlights significant gene regulation differences. (B) Gene set enrichment analysis (GSEA) shows that DEGs are primarily involved in immune regulation pathways. (C) Heatmap of gene expression reveals the upregulation and downregulation of genes associated with diabetic wound healing in the LZM-HZ-10 hydrogels compared to the blank. (D) Gene Ontology (GO) enrichment analysis barplot illustrates the assessment enriched biological processes, with bar length indicating the number of genes and color representing the significance level (lighter colors denote higher significance). (E) KEGG pathway enrichment bubble chart showing the distribution of gene enrichment differences within pathways. While color reflects the p-value, with lighter shades indicating higher significance. (F) Representative Western blot bands of phosphorylated STAT3 (p-STAT3), phosphorylated <t>STAT6</t> (p-STAT6), and Arg1 in wound tissues from diabetic mice treated with LZM-HZ-10 hydrogel, LZM-SC hydrogel and Blank. (G–I) Quantitative analysis of relative protein expression levels normalized to GAPDH. Increased levels of p-STAT3, p-STAT6, and Arg1 in the LZM-HZ-10 hydrogel confirm activation of the JAK-STAT signaling pathway at the protein level. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.)
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Mechanistic exploration of diabetic wound healing facilitated by LZM-HZ hydrogels. (A) Volcano plot of differentially expressed genes (DEGs) between the LZM-HZ-10 hydrogels and blank on Day 7 highlights significant gene regulation differences. (B) Gene set enrichment analysis (GSEA) shows that DEGs are primarily involved in immune regulation pathways. (C) Heatmap of gene expression reveals the upregulation and downregulation of genes associated with diabetic wound healing in the LZM-HZ-10 hydrogels compared to the blank. (D) Gene Ontology (GO) enrichment analysis barplot illustrates the assessment enriched biological processes, with bar length indicating the number of genes and color representing the significance level (lighter colors denote higher significance). (E) KEGG pathway enrichment bubble chart showing the distribution of gene enrichment differences within pathways. While color reflects the p-value, with lighter shades indicating higher significance. (F) Representative Western blot bands of phosphorylated STAT3 (p-STAT3), phosphorylated STAT6 (p-STAT6), and Arg1 in wound tissues from diabetic mice treated with LZM-HZ-10 hydrogel, LZM-SC hydrogel and Blank. (G–I) Quantitative analysis of relative protein expression levels normalized to GAPDH. Increased levels of p-STAT3, p-STAT6, and Arg1 in the LZM-HZ-10 hydrogel confirm activation of the JAK-STAT signaling pathway at the protein level. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.)

Journal: Bioactive Materials

Article Title: Catalyst-modulated hydrogel dynamics for decoupling viscoelasticity and directing macrophage fate for diabetic wound healing

doi: 10.1016/j.bioactmat.2025.06.007

Figure Lengend Snippet: Mechanistic exploration of diabetic wound healing facilitated by LZM-HZ hydrogels. (A) Volcano plot of differentially expressed genes (DEGs) between the LZM-HZ-10 hydrogels and blank on Day 7 highlights significant gene regulation differences. (B) Gene set enrichment analysis (GSEA) shows that DEGs are primarily involved in immune regulation pathways. (C) Heatmap of gene expression reveals the upregulation and downregulation of genes associated with diabetic wound healing in the LZM-HZ-10 hydrogels compared to the blank. (D) Gene Ontology (GO) enrichment analysis barplot illustrates the assessment enriched biological processes, with bar length indicating the number of genes and color representing the significance level (lighter colors denote higher significance). (E) KEGG pathway enrichment bubble chart showing the distribution of gene enrichment differences within pathways. While color reflects the p-value, with lighter shades indicating higher significance. (F) Representative Western blot bands of phosphorylated STAT3 (p-STAT3), phosphorylated STAT6 (p-STAT6), and Arg1 in wound tissues from diabetic mice treated with LZM-HZ-10 hydrogel, LZM-SC hydrogel and Blank. (G–I) Quantitative analysis of relative protein expression levels normalized to GAPDH. Increased levels of p-STAT3, p-STAT6, and Arg1 in the LZM-HZ-10 hydrogel confirm activation of the JAK-STAT signaling pathway at the protein level. (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.)

Article Snippet: The following primary antibodies were used: p-STAT3 (ab76315, Abcam), STAT3 (ab68153, Abcam), p-STAT6 (#9361, Cell Signaling Technology), STAT6 (#5397, Cell Signaling Technology), Arg1 (16001-1-AP, Proteintech), and GAPDH (AC002, ABclonal).

Techniques: Gene Expression, Western Blot, Expressing, Activation Assay